guide-it complete sgrna screening system

来自 : www.takarabiomed.com.cn/Produc 发布时间：2021-03-24

Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635; 50 rxns)
· 1 each Guide-it sgRNA In Vitro Transcription Components v2 (Code No. 632637; 50 rxns; Not sold separately)
○ 50 μl Guide-it Scaffold Template (1 ng/μl)
○ 350 μl Guide-itIn VitroTranscription Buffer
○ 1 ml RNase Free Water
○ 500 μl RNase Free Water
○ 150 μl Guide-it T7 Polymerase Mix (33 U/μl)
○ 625 μl PrimeSTAR Max Premix (2X)
○ 100 μl Recombinant DNase I (RNase-Free) (5 U/μl)
· 1 each Guide-it IVT RNA Clean-Up Kit (Code No. 632638; 50 rxns)
○ 13 ml RNase Free Water
○ 3 ml IVT Binding Buffer
○ 50 IVT RNA Clean-Up Spin Columns
○ 2 x 6 ml IVT Wash Buffer
○ 50 Collection Tubes (2 ml)
Guide-it sgRNA Clean-Up Kit (Code No. 632638; 50 rxns)
· 13 ml RNase Free Water
· 3 ml IVT Binding Buffer
· 50 IVT RNA Clean-Up Spin Columns
· 2 x 6 ml IVT Wash Buffer
· 50 Collection Tubes (2 ml)
Guide-it sgRNA Screening Kit (Code No. 632639; 50 rxns)
· 25 μl Guide-it Recombinant Cas9 Nuclease (500 ng/μl)
· 50 μl 15X Cas9 Reaction Buffer
· 50 μl 15X BSA
· 5 μl Control sgRNA (50 ng/μl)
· 25 μl Control Fragment (20 ng/μl)
· 50 μl Terra PCR Direct Polymerase Mix (1.25 U/μl)
· 1.25 ml 2X Terra PCR Direct Buffer (with Mg2+, dNTP)
· 5 ml RNase Free Water
· 5 ml Extraction Buffer 1
· 0.5 ml Extraction Buffer 2

Guide-it Complete sgRNA Screening System(Code No. 632636; 50 rxns)
· Guide-it sgRNA In Vitro Transcription Kit (Code No. 632635)
· Guide-it sgRNA Screening Kit (Code No. 632639)